IOCB Prague

Mass Spectrometry of Biopolymers

Karel Harant Group
Core Facility
BIO cluster

About our group

The Mass Spectrometry of Biopolymers (BioMS) provides analytical services for proteins and other biomolecules based on mass spectrometric detection. We provide identification, characterization, and quantification of purified proteins, bands from SDS-PAGE, imunoprecipitation experiments, cell lysates and more. We are also capable of analyzing posttranslational modifications of proteins including global phosphoproteomes. We are currently developing complementary methods for the analysis of nucleic acids and their complexes with proteins. In addition to routinely used proteomic techniques, we are also implementing structural mass spectrometry methods for analysis of protein-protein and protein-ligand interactions, such as top-down native mass analysis of protein complexes, protein chemical crosslinking, and hydrogen-deuterium exchange mass spectrometry (HDX-MS).

You can find more information on individual analysis types available on the Service page.

The service facility is currently undergoing a restructuring.